Benzodiazepines drug profile

Benzodiazepines drug profile

Benzodiazepines are widely used in medicine to treat anxiety and insomnia. These are synthetic substances normally seen as pharmaceutically-manufactured tablets, capsules and occasionally as injectables. They act as depressants of the central nervous system (CNS). Chlordiazepoxide (Librium) was the first to be synthesised in 1957 and introduced into medicine in 1961. Benzodiazepines are under international control.


The fully systematic (IUPAC) name for the nucleus of the benzodiazepine group (CAS 12794-10-4) is 2,3-diazabicyclo[5.4.0]undeca-3,5,7,9,11pentaene. The different drugs have varying substituents on this basic skeleton.
Diazepam (CAS 439-14-5) is one of the best known benzodiazepines (Valium). According to IUPAC, the fully systematic name is 9-chloro-2-methyl-6-phenyl-2,5-diazabicyclo[5.4.0]undeca-5,8, 10,12-tetraen-3-one or 7-chloro-1,3-dihydro-1-methyl-5-phenyl-2H-1,4-benzodiazepine-2-one.

Molecular structure: Diazepam

molecular structure of diazepam

Molecular formula: C16H13CIN2O
Molecular weight: 284.7

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Physical form

Tablets, capsules, injectables (e.g. diazepam, lorazepam, midazolam), suppositories.

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Benzodiazepines are a group of CNS depressants which induce feelings of calm (anxiolysis), drowsiness and sleep. They act by facilitating the binding of the inhibitory neurotransmitter GABA at various GABA receptors throughout the CNS. Because they have a lower tendency to cause a potentially fatal CNS depression compared to earlier drugs such as barbiturates, benzodiazepines are widely used in medicine for the treatment of anxiety (anxiolytics) and insomnia (sedative/hypnotics), as well as other psychological conditions such as panic attacks and panic disorders. There is no clear division between anxiolytics and hypnotics, since most anxiolytics will induce sleep if taken at night and most hypnotics will sedate when taken during the day.

Different benzodiazepines vary in the rate in which they are metabolised to pharmacologically active forms and particularly in their half-lives (see Table 1): short-acting drugs have a half-life of less than 24 hours e.g. midazolam; intermediate-acting compounds such as nitrazepam have half-lives greater than 24 hours, whereas long-acting compounds such as diazepam have half-lives greater than 48 hours. Such half-lives vary between individuals, and the elderly tend to eliminate these drugs much more slowly. They are thus more at risk from the side-effects which include drowsiness, ataxia (staggering gait), mental confusion, impaired judgement and anterograde amnesia. There is a significantly increased risk of adverse events in the elderly such as falls, diminished cognitive function and driving impairment, although the latter is not confined to the elderly. The European prevalence studies show that, excluding alcohol, benzodiazepines are along with cannabis the psychoactive substances most prevalent in the driving population. Experimental studies show that these drugs impair driving ability and when alcohol is also used, the risk of being involved in or responsible for a road accident is significantly increased.

Benzodiazepine intoxication can be associated with behavioural disinhibition, potentially resulting in hostile or aggressive behaviour. The effect is perhaps most common when benzodiazepines are taken in combination with alcohol. The combined use of alcohol and benzodiazepines also increases the risk of a fatal overdose because both act as CNS depressants. A similar fatal interaction can occur when opiates are taken with benzodiazepines as part of a pattern of polydrug use. A significant number of problem drug users swallow, ‘snort’ or inject high doses of benzodiazepines to enhance the euphoriant effects of opiates or to minimise unpleasant effects of psychostimulants. The EMCDDA’s Annual report on the state of the drugs problem in Europe highlights the fact that concomitant use of benzodiazepines and opiates is a major risk factor in drug-related deaths. Apart from the increased risk of fatal overdoses, the usual injection-specific diseases such as tissue damage, gangrene and transmission of HIV and hepatitis C also occur if the drugs are injected.

There is also the risk of cross-dependence developing to benzodiazepines. Medically, benzodiazepines should only be used for the short-term relief of anxiety or insomnia which is severe and disabling. This is because tolerance and dependence can occur just weeks after use has commenced. Withdrawal signs and symptoms can be classified as major or minor, like those of the alcohol syndrome. According to that classification, minor symptoms include anxiety, insomnia and nightmares. Major symptoms include perceptual disturbances, psychosis, hyperpyrexia and life-threatening convulsions.

Table 1: List of benzodiazepines under international control

Name Duration of action Major trade name CAS No


Brotizolam Short Lendormin 57801-81-7
Estazolam Intermediate Pro-Som 29975-16-4
Flunitrazepam Short/Intermediate Rohypnol 1622-62-4
Flurazepam Long Dalmane 17617-23-1
Haloxazolam Long Somelin 59128-97-1
Loprazolam Intermediate Dormonoct 61197-73-7
Lormetazepam Short Noctamid 848-75-9
Midazolam Short Versed 59467-70-8
Nimetazepam Long Erinin 2011-67-8
Nitrazepam Intermediate Mogadon 146-22-5
Temazepam Short Normison 846-50-4
Triazolam Short Halcion 28911-01-5


Alprazolam Short Xanax 20981-97-7
Bromazepam Long Lexotan 1812-30-2
Camazepam   Albego 36104-80-2
Chlordiazepoxide Long Librium 438-41-5
Clobazam Long Frisium 22316-47-8
Clonazepam Intermediate Rivotril 1622-61-3
Clorazepate Long Tranxene 57109-90-7
Clotiazepam Short Trecalmo 33671-46-7
Cloxazolam Long Sepazon 24166-13-0
Delorazepam Long En 2894-67-9
Diazepam Long Valium 439-14-5
Ethyl hoflazepate Long Meilax 29177-84-2
Fludiazepam Short Erispan 3900-31-00
Halazepam Long Pacinone 23092-17-3
Ketazolam Long Anseren 27223-49-1
Lorazepam Short/Intermediate Ativan 846-49-1
Medazepam Long Nobrium 2898-12-6
Nordazepam Long Stilny 1088-11-5
Oxazepam Short Serax 604-75-1
Oxazolam Long Tranquit 27167-30-2
Pinazepam Long Domar 52463-83-9
Prazepam Long Centrax 2955-38-6
Tetrazepam Short Clinoxan 10379-14-3

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Benzodiazepines, sexual assaults and other crimes

Flunitrazepam (Rohypnol) is the benzodiazepine most commonly linked by media reports to drug-facilitated sexual assaults, more commonly referred to as ‘date rape’. However, forensic toxicology shows that only a very small number of such assaults actually involve the use of flunitrazepam. A number of studies, cited in the EMCDDA Technical data sheet on Sexual assaults facilitated by drugs or alcohol (EMCDDA, 2008), suggest that alcohol and other benzodiazepines are an underestimated problem in such cases. Other work has identified covert use of benzodiazepines to facilitate theft.  Forensic analysis cannot unequivocally distinguish between the deliberately covert ‘spiking’ of drinks, and voluntary or prescribed use of benzodiazepines, however some forms of hair analysis can distinguish regular long-term use from a single ingestion.

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The synthesis of benzodiazepines is performed by the pharmaceutical and chemical industry, often using patented methods. While the vast bulk of benzodiazepines are manufactured pharmaceutically, information on Internet websites describes a series of routes to producing diazepam using 5-chloro-N-methyl-isatoicanhydride and 2-amino-5-chlorobenzophenone as precursors.

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Mode of use

Benzodiazepines are usually swallowed as tablets but can be injected for both medical and non-medical purposes and there are some reports of intranasal (snorting) misuse.

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Other names

Numerous synonyms and proprietary names exist for the 35 benzodiazepines under international control. When originally introduced, they were misleadingly referred to as ‘minor’ tranquillisers to distinguish them from ‘major’ tranquillisers used as anti-psychotics.

Users’ terms include: benzos, blues/blueys, tranx, roche’s, mother's little helpers, duck eggs (temazepam), roofies (Rohypnol), V’s, and many others.

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Most benzodiazepines including diazepam give an orange colour with a mixture of formaldehyde and concentrated sulphuric acid when heated at 100° C for 1 minute (this is a variant of the Marquis test, where heat is applied). Flunitrazepam gives a pink colour.  Benzodiazepines also give a reddish-purple or pink colour in the Zimmerman test. The mass spectrum for diazepam shows a major ion at m/z = 256 and others at 283, 284, 285, 257, 255, 258 and 286.  The major ions for temazepam (molecular weight 300.7) are at 271, 273, 300 and 272, whereas those for flunitrazepam (molecular weight 313.28) are at 285, 312, 313 and 286. Using gas chromatography–mass spectrometry the limit of detection for diazepam in blood is 0.2–20 µg/L. With high performance liquid chromatography–mass spectrometry the limit in serum or urine is 2 µg/L.

Typical therapeutic concentrations are 0.1–1.0 mg/L, while toxic effects occur at levels of  more than 1.5 mg/L. Fatalities (rare where only one drug is involved) may occur at levels in excess of 5 mg/L.

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Control status

Thirty-three benzodiazepines were included in Schedule IV of the 1971 United Nations Convention on Psychotropic Substances in 1984 (Table 1). Midazolam (1990) and brotizolam (1995) were subsequently added to the Schedule. In 1995, flunitrazepam (CAS 1622-62-4) was transferred from Schedule IV to Schedule III because the International  Narcotics Control Board (INCB) stated that it was one of the most misused benzodiazepines and because of its frequent diversion into the illicit market.

Phenazepam (fenazepam) (CAS 51753-57-2), which is used in medical practice in some countries outside of the European Union, is not scheduled in the 1971 United Nations Convention on Psychotropic Substances.

Availability of pharmaceutical benzodiazepines

The INCB reported that in 2006, total global licit production of benzodiazepines amounted to at least 180 metric tonnes, 56 tonnes of which was diazepam. Italy (32 %), India (19 %), China (11 %) and Germany (10 %) were the leading manufacturers between 1997 and 2006.

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