This Reitox Academy was organised by the EMCDDA in close cooperation with the Hungarian Institute for Forensic Sciences and the Hungarian national focal point. The objective of the training was to provide participants with knowledge on how to detect and analyse most common groups of new psychoactive substance (NPS; protocols, methods or networks for generation of standard samples), and to discuss the role of forensic laboratories within the national EWS. Fifteen experts from all six countries participating in the EMCDDA IPA5 project (2015–17) got a further insight into the criteria and diverse requirements to be met when performing an NPS analysis, discussed the chemistry of NPS and international guidelines and available analytical techniques for their identification in seized and biological materials. The participants also visited laboratories to familiarise themselves with the facilities, equipment and methodologies used in this field and encouraged discussions on possible future developments. The majority of participants were satisfied with the results of the training and the opportunity to share experiences and to interact with other experts and trainers.